Review




Structured Review

Synthego Inc sgrna database
Sgrna Database, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+database/database+sgrna/10__7554_slash_elife__101170__3-369-35-39
Average 86 stars, based on 1 article reviews
sgrna database - by Bioz Stars, 2026-11
86/100 stars

Images

Related Articles

Knock-Out:

Article Title: Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing
Article Snippet: .. To generate RYBP and AFF1 knock out TOs line, two sets of sgRNA pairs for each gene with the target sites close to the 5 prime of genes ORF were selected from a pre- designed sgRNA database established by Synthego (https://design.synthego.com/#/), then each sgRNA pair (dual- sgRNAs) were cloned into individual cassettes on Lentiviral transfer plasmid with Cas9 ORF (Dual- gRNA lentivirus CRISPR vector). ..

Clone Assay:

Article Title: Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing
Article Snippet: .. To generate RYBP and AFF1 knock out TOs line, two sets of sgRNA pairs for each gene with the target sites close to the 5 prime of genes ORF were selected from a pre- designed sgRNA database established by Synthego (https://design.synthego.com/#/), then each sgRNA pair (dual- sgRNAs) were cloned into individual cassettes on Lentiviral transfer plasmid with Cas9 ORF (Dual- gRNA lentivirus CRISPR vector). ..

Plasmid Preparation:

Article Title: Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing
Article Snippet: .. To generate RYBP and AFF1 knock out TOs line, two sets of sgRNA pairs for each gene with the target sites close to the 5 prime of genes ORF were selected from a pre- designed sgRNA database established by Synthego (https://design.synthego.com/#/), then each sgRNA pair (dual- sgRNAs) were cloned into individual cassettes on Lentiviral transfer plasmid with Cas9 ORF (Dual- gRNA lentivirus CRISPR vector). ..

CRISPR:

Article Title: Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing
Article Snippet: .. To generate RYBP and AFF1 knock out TOs line, two sets of sgRNA pairs for each gene with the target sites close to the 5 prime of genes ORF were selected from a pre- designed sgRNA database established by Synthego (https://design.synthego.com/#/), then each sgRNA pair (dual- sgRNAs) were cloned into individual cassettes on Lentiviral transfer plasmid with Cas9 ORF (Dual- gRNA lentivirus CRISPR vector). ..



Similar Products

86
Synthego Inc sgrna database
Sgrna Database, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+database/database+sgrna/10__7554_slash_elife__101170__3-369-35-39
Average 86 stars, based on 1 article reviews
sgrna database - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

90
GenScript corporation crispr sgrna database
Crispr Sgrna Database, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+database/crispr+sgrna+database/pm40232306-173-11-15
Average 90 stars, based on 1 article reviews
crispr sgrna database - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
GenScript corporation broad sgrna database
Broad Sgrna Database, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+database/broad+sgrna+database/pmc11178546-190-15-14
Average 90 stars, based on 1 article reviews
broad sgrna database - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
GenScript corporation sgrna database
Troubleshooting table
Sgrna Database, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+database/sgrna+library/pmc09059808-469-9-12
Average 90 stars, based on 1 article reviews
sgrna database - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Troubleshooting table

Journal: Nature protocols

Article Title: Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

doi: 10.1038/s41596-021-00669-0

Figure Lengend Snippet: Troubleshooting table

Article Snippet: To identify an sgRNA target sequence using the GenScript sgRNA database ( https://www.genscript.com/gRNA-database.html ) or other webtools, input the gene name, symbol or ID and search for human SpCas9 gRNA sequences.

Techniques: Negative Control, Ligation, Transformation Assay, Plasmid Preparation, Selection, Passaging, Clone Assay, Concentration Assay, Sequencing